DBNascent | Samples

SRM497912 Sample ID 930

**Data sources**
Paper identifier	Hah2013enhancer
SRP accession	SRP018256
GEO accession	GSE43835
Samples	SRR653424
	SRR497912
	SRR497913

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	113 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol (100 nM)	-10 min	0 min	10 min

**Extended notes**
Pipeline info and software versions
Sample notes	This is a resequencing tech rep for SRR497912-SRR497913, published in Hah2011rapid
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	40
Read depth after trimming	52,235,114
Duplication proportion	0.66429
Proportion reads mapped	0.8022
Exon/intron ratio	2.77734
Unique read proportion	0.56509

**Bidirectionals**
Total # Tfit bidirectionals	38875
# Promoter Tfit bidirectionals	9380
# Intronic Tfit bidirectionals	19699
# Intergenic Tfit bidirectionals	8059
Total # dREG bidirectionals	27862
# Promoter dREG bidirectionals	9351
# Intronic dREG bidirectionals	12137
# Intergenic dREG bidirectionals	6108
Part of Tfit master merge?	True
Part of dREG master merge?	True