DBNascent | Samples

SRM497914 Sample ID 931

**Data sources**
Paper identifier	Hah2013enhancer
SRP accession	SRP018256
GEO accession	GSE43835
Samples	SRR653425
	SRR497914
	SRR497915
	SRR497916

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	112 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	4 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol (100 nM)	-40 min	0 min	40 min

**Extended notes**
Pipeline info and software versions
Sample notes	This is a resequencing tech rep for SRR497914-SRR497916, published in Hah2011rapid
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	40
Read depth after trimming	46,484,517
Duplication proportion	0.6337
Proportion reads mapped	0.8465
Exon/intron ratio	3.12937
Unique read proportion	0.57874

**Bidirectionals**
Total # Tfit bidirectionals	41935
# Promoter Tfit bidirectionals	10035
# Intronic Tfit bidirectionals	20678
# Intergenic Tfit bidirectionals	9356
Total # dREG bidirectionals	31072
# Promoter dREG bidirectionals	9576
# Intronic dREG bidirectionals	14052
# Intergenic dREG bidirectionals	7088
Part of Tfit master merge?	True
Part of dREG master merge?	True