DBNascent | Samples

SRR1015593 Sample ID 1758

**Data sources**
Paper identifier	Luo2014dynamic
SRP accession	SRP031852
GEO accession	GSE51224
Samples	SRR1015593

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	142 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	AC16
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Nuclei treated with 1 microg/mL alpha-amanitin for 15 min before run-on
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	51
Read depth after trimming	129,665,819
Duplication proportion	0.7992
Proportion reads mapped	0.8304
Exon/intron ratio	1.7294
Unique read proportion	0.27885

**Bidirectionals**
Total # Tfit bidirectionals	39018
# Promoter Tfit bidirectionals	14036
# Intronic Tfit bidirectionals	15648
# Intergenic Tfit bidirectionals	8667
Total # dREG bidirectionals	27155
# Promoter dREG bidirectionals	9255
# Intronic dREG bidirectionals	11052
# Intergenic dREG bidirectionals	6740
Part of Tfit master merge?	True
Part of dREG master merge?	True