DBNascent | Samples

SRR1015594 Sample ID 1759

**Data sources**
Paper identifier	Luo2014dynamic
SRP accession	SRP031852
GEO accession	GSE51224
Samples	SRR1015594

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	122 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	4 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	AC16
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Nuclei treated with 1 microg/mL alpha-amanitin for 15 min before run-on
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	51
Read depth after trimming	153,544,155
Duplication proportion	0.86384
Proportion reads mapped	0.8547
Exon/intron ratio	1.5369
Unique read proportion	0.2072

**Bidirectionals**
Total # Tfit bidirectionals	41096
# Promoter Tfit bidirectionals	14077
# Intronic Tfit bidirectionals	17613
# Intergenic Tfit bidirectionals	8108
Total # dREG bidirectionals	24615
# Promoter dREG bidirectionals	8719
# Intronic dREG bidirectionals	9665
# Intergenic dREG bidirectionals	5946
Part of Tfit master merge?	True
Part of dREG master merge?	True