DBNascent | Samples

SRR10312750 Sample ID 1491

**Data sources**
Paper identifier	Li2020human
SRP accession	SRP226233
GEO accession	GSE139114
Samples	SRR10312750

**Technical details**
QC score	3
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	38 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	1001 Kb
dREG bidirectional regions	BED file	267 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	primary cell
Cell type	foreskin fibroblast
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	HCMV strain Towne IE2 BAC (MOI 3)	-96 hr	0 hr	4 day
treatment	dTag7 (200 nM)	-6 hr	0 hr	6 hr
treatment	flavopiridol (1 microM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	HCMV strains have different genes tagged with degron
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	151
Read depth after trimming	27,986,259
Duplication proportion	0.58077
Proportion reads mapped	0.4429
Exon/intron ratio	3.84498
Unique read proportion	0.26092

**Bidirectionals**
Total # Tfit bidirectionals	11525
# Promoter Tfit bidirectionals	7108
# Intronic Tfit bidirectionals	4211
# Intergenic Tfit bidirectionals	642
Total # dREG bidirectionals	6228
# Promoter dREG bidirectionals	4907
# Intronic dREG bidirectionals	1498
# Intergenic dREG bidirectionals	410
Part of Tfit master merge?	True
Part of dREG master merge?	True