DBNascent | Samples

SRR10312758 Sample ID 1499

**Data sources**
Paper identifier	Li2020human
SRP accession	SRP226233
GEO accession	GSE139114
Samples	SRR10312758

**Technical details**
QC score	5
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	28 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	389 Kb
dREG bidirectional regions	BED file	20 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	primary cell
Cell type	foreskin fibroblast
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	HCMV strain TB40/E IE2F (MOI 3)	-72 hr	0 hr	3 day
treatment	dTag7 (200 nM)	-6 hr	0 hr	6 hr

**Extended notes**
Pipeline info and software versions
Sample notes	HCMV strains have different genes tagged with degron
Analysis notes

**Sample metrics**
Sample QC score	5
Sample NRO score	2
Raw read length	151
Read depth after trimming	25,975,978
Duplication proportion	0.35778
Proportion reads mapped	0.1263
Exon/intron ratio	3.20455
Unique read proportion	0.48307

**Bidirectionals**
Total # Tfit bidirectionals	4401
# Promoter Tfit bidirectionals	1831
# Intronic Tfit bidirectionals	1671
# Intergenic Tfit bidirectionals	405
Total # dREG bidirectionals	452
# Promoter dREG bidirectionals	233
# Intronic dREG bidirectionals	110
# Intergenic dREG bidirectionals	102
Part of Tfit master merge?	True
Part of dREG master merge?	True