DBNascent | Samples

SRR10312760 Sample ID 1501

**Data sources**
Paper identifier	Li2020human
SRP accession	SRP226233
GEO accession	GSE139114
Samples	SRR10312760

**Technical details**
QC score	4
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	46 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	919 Kb
dREG bidirectional regions	BED file	85 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	primary cell
Cell type	foreskin fibroblast
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	HCMV strain TB40/E IE2F (MOI 3)	-72 hr	0 hr	3 day
treatment	dTag7 (200 nM)	-6 hr	0 hr	6 hr
treatment	phosphonoformic acid (400 microg/mL)	-6 hr	0 hr	6 hr

**Extended notes**
Pipeline info and software versions
Sample notes	HCMV strains have different genes tagged with degron
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	151
Read depth after trimming	39,194,105
Duplication proportion	0.28535
Proportion reads mapped	0.1534
Exon/intron ratio	2.98158
Unique read proportion	0.5768

**Bidirectionals**
Total # Tfit bidirectionals	10399
# Promoter Tfit bidirectionals	4804
# Intronic Tfit bidirectionals	4143
# Intergenic Tfit bidirectionals	844
Total # dREG bidirectionals	1983
# Promoter dREG bidirectionals	1176
# Intronic dREG bidirectionals	615
# Intergenic dREG bidirectionals	221
Part of Tfit master merge?	True
Part of dREG master merge?	True