DBNascent | Samples

SRR10400222 Sample ID 2640

**Data sources**
Paper identifier	Wang2020proapoptotic
SRP accession	SRP228576
GEO accession	GSE139928
Samples	SRR10400222

**Technical details**
QC score	1
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	107 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	550 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype	dox-inducible Pax2 (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	doxycycline (50 ng/mL)	-22 hr	0 hr	22 hr
treatment	hydroxytamoxifen (1 microM)	-6 hr	0 hr	6 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Vehicle unspecified
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	1
Raw read length	51
Read depth after trimming	28,160,127
Duplication proportion	0.23499
Proportion reads mapped	0.7859
Exon/intron ratio	1.76367
Unique read proportion	0.80208

**Bidirectionals**
Total # Tfit bidirectionals	20215
# Promoter Tfit bidirectionals	7556
# Intronic Tfit bidirectionals	8891
# Intergenic Tfit bidirectionals	3081
Total # dREG bidirectionals	12721
# Promoter dREG bidirectionals	5993
# Intronic dREG bidirectionals	4793
# Intergenic dREG bidirectionals	2092
Part of Tfit master merge?	True
Part of dREG master merge?	True