DBNascent | Samples

SRR10439770 Sample ID 2806

**Data sources**
Paper identifier	Zhang2020bcatenin
SRP accession	SRP229740
GEO accession	GSE140340
Samples	SRR10439770

**Technical details**
QC score	5
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	11 Mb
Stranded gene read counts	TXT file	893 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	167 Kb
dREG bidirectional regions	BED file	3 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	PD0325901 (1 microM)	None	None	None
treatment	CHIR99021 (3 microM)	None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Length of culture not specified; PD0325901=MEK inhibitor; CHIR99021=Gsk3 inhibitor; Not much library prep info, but mentions polyA tailing reads
Analysis notes

**Sample metrics**
Sample QC score	5
Sample NRO score	1
Raw read length	150
Read depth after trimming	24,894,386
Duplication proportion	0.66613
Proportion reads mapped	0.0602
Exon/intron ratio	1.7037
Unique read proportion	0.19276

**Bidirectionals**
Total # Tfit bidirectionals	1929
# Promoter Tfit bidirectionals	704
# Intronic Tfit bidirectionals	523
# Intergenic Tfit bidirectionals	379
Total # dREG bidirectionals	64
# Promoter dREG bidirectionals	6
# Intronic dREG bidirectionals	22
# Intergenic dREG bidirectionals	34
Part of Tfit master merge?	True
Part of dREG master merge?	True