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SRR10439771 Sample ID 2807
| Paper identifier | Zhang2020bcatenin |
| SRP accession | SRP229740 |
| GEO accession | GSE140340 |
| Samples | SRR10439771 |
| QC score | 5 |
| Replicate | 2 |
| Protocol | GRO-seq |
| Library prep | circularization |
| Spike-in | None |
| Single/Paired end | paired |
| Raw strandedness | read1 forward |
| Reverse complemented? | True |
| Mapped strandedness | read1 forward |
| Timecourse | False |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | 14 Mb |
| Stranded gene read counts | TXT file | 896 Kb |
| Unstranded bidirectional read counts | TXT file | 17 Mb |
| Tfit bidirectional regions | BED file | 208 Kb |
| dREG bidirectional regions | BED file | 3 Kb |
| All data files | ZIP archive | |
| Organism | M. musculus |
| Sample type | cell line |
| Cell type | ESC |
| Clone/Individual | |
| Strain | |
| Genotype | |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | PD0325901 (1 microM) | None | None | None |
| treatment | CHIR99021 (3 microM) | None | None | None |
| Pipeline info and software versions | |
| Sample notes | Length of culture not specified; PD0325901=MEK inhibitor; CHIR99021=Gsk3 inhibitor; Not much library prep info, but mentions polyA tailing reads |
| Analysis notes |
| Sample QC score | 5 |
| Sample NRO score | 1 |
| Raw read length | 150 |
| Read depth after trimming | 20,486,015 |
| Duplication proportion | 0.67452 |
| Proportion reads mapped | 0.0934 |
| Exon/intron ratio | 1.74286 |
| Unique read proportion | 0.19742 |
| Total # Tfit bidirectionals | 2392 |
| # Promoter Tfit bidirectionals | 663 |
| # Intronic Tfit bidirectionals | 680 |
| # Intergenic Tfit bidirectionals | 483 |
| Total # dREG bidirectionals | 79 |
| # Promoter dREG bidirectionals | 6 |
| # Intronic dREG bidirectionals | 28 |
| # Intergenic dREG bidirectionals | 43 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |