SRR10486680 Sample ID 1939

Data sources
Paper identifier Nguyen2020dichotomous
SRP accession SRP230423
GEO accession GSE140573
Samples SRR10486680
Technical details
QC score 2
Replicate 1
Protocol GRO-seq
Library prep circularization
Spike-in None
Single/Paired end single
Raw strandedness forward
Reverse complemented? False
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file 62 Mb
Stranded gene read counts TXT file 914 Kb
Unstranded bidirectional read counts TXT file 17 Mb
dREG bidirectional regions BED file 570 Kb
All data files ZIP archive
Cellular details
Organism M. musculus
Sample type primary cell
Cell type bone marrow derived macrophage
Clone/Individual
Strain
Genotype Mhd3 -/-
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment lipopolysaccharide (5 ng/mL) -4 hr 0 hr 4 hr
Extended notes
Pipeline info and software versions
Sample notes
Analysis notes Poor quality libraries, many are mostly poly-A or unknown contamination
Sample metrics
Sample QC score 2
Sample NRO score 1
Raw read length 100
Read depth after trimming 65,118,397
Duplication proportion 0.52124
Proportion reads mapped 0.2596
Exon/intron ratio 1.35868
Unique read proportion 0.56423
Bidirectionals
Total # Tfit bidirectionals None
# Promoter Tfit bidirectionals None
# Intronic Tfit bidirectionals None
# Intergenic Tfit bidirectionals None
Total # dREG bidirectionals 13229
# Promoter dREG bidirectionals 4443
# Intronic dREG bidirectionals 4693
# Intergenic dREG bidirectionals 3314
Part of Tfit master merge? False
Part of dREG master merge? False