SRR10486682 Sample ID 1941

Data sources
Paper identifier Nguyen2020dichotomous
SRP accession SRP230423
GEO accession GSE140573
Samples SRR10486682
Technical details
QC score 3
Replicate 3
Protocol GRO-seq
Library prep circularization
Spike-in None
Single/Paired end single
Raw strandedness forward
Reverse complemented? False
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file 136 Mb
Stranded gene read counts TXT file 926 Kb
Unstranded bidirectional read counts TXT file 17 Mb
dREG bidirectional regions BED file 1 Mb
All data files ZIP archive
Cellular details
Organism M. musculus
Sample type primary cell
Cell type bone marrow derived macrophage
Clone/Individual
Strain
Genotype Mhd3 -/-
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment lipopolysaccharide (5 ng/mL) -4 hr 0 hr 4 hr
Extended notes
Pipeline info and software versions
Sample notes
Analysis notes Poor quality libraries, many are mostly poly-A or unknown contamination
Sample metrics
Sample QC score 3
Sample NRO score 1
Raw read length 50
Read depth after trimming 147,467,903
Duplication proportion 0.65277
Proportion reads mapped 0.3836
Exon/intron ratio 1.84203
Unique read proportion 0.56594
Bidirectionals
Total # Tfit bidirectionals None
# Promoter Tfit bidirectionals None
# Intronic Tfit bidirectionals None
# Intergenic Tfit bidirectionals None
Total # dREG bidirectionals 29221
# Promoter dREG bidirectionals 6899
# Intronic dREG bidirectionals 12271
# Intergenic dREG bidirectionals 8451
Part of Tfit master merge? False
Part of dREG master merge? False