DBNascent | Samples

SRR10502417 Sample ID 260

**Data sources**
Paper identifier	Birkenheuer2020rna
SRP accession	SRP193891
GEO accession	GSE130342
Samples	SRR10502417

**Technical details**
QC score	2
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	D. melanogaster
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	80 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	399 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEp2
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	HSV-1 (MOI 5)	-6 hr	-5 hr	1 hr
treatment	acyclovir (10 microM)	-5 hr	0 hr	5 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	35
Read depth after trimming	26,912,039
Duplication proportion	0.38589
Proportion reads mapped	0.5882
Exon/intron ratio	4.08354
Unique read proportion	0.63097

**Bidirectionals**
Total # Tfit bidirectionals	20119
# Promoter Tfit bidirectionals	6401
# Intronic Tfit bidirectionals	8502
# Intergenic Tfit bidirectionals	4473
Total # dREG bidirectionals	9232
# Promoter dREG bidirectionals	4340
# Intronic dREG bidirectionals	3056
# Intergenic dREG bidirectionals	1880
Part of Tfit master merge?	True
Part of dREG master merge?	True