SRR10502421 Sample ID 264

Data sources
Paper identifier Birkenheuer2020rna
SRP accession SRP193891
GEO accession GSE130342
Samples SRR10502421
Technical details
QC score 3
Replicate 1
Protocol PRO-seq
Library prep ligation
Spike-in D. melanogaster
Single/Paired end single
Raw strandedness reverse
Reverse complemented? True
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type HEp2
Clone/Individual
Strain
Genotype
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment HSV-1 (MOI 5) -6 hr -5 hr 1 hr
treatment flavopiridol (450 nM) -6 hr 0 hr 6 hr
Extended notes
Pipeline info and software versions
Sample notes length of flavopiridol treatment unclear - could be 1 hr or 6 hr
Analysis notes
Sample metrics
Sample QC score 3
Sample NRO score 5
Raw read length 35
Read depth after trimming 27,309,578
Duplication proportion 0.76584
Proportion reads mapped 0.6715
Exon/intron ratio 14.3402
Unique read proportion 0.28009
Bidirectionals
Total # Tfit bidirectionals 24836
# Promoter Tfit bidirectionals 9356
# Intronic Tfit bidirectionals 10782
# Intergenic Tfit bidirectionals 4951
Total # dREG bidirectionals 20228
# Promoter dREG bidirectionals 8669
# Intronic dREG bidirectionals 8268
# Intergenic dREG bidirectionals 3539
Part of Tfit master merge? True
Part of dREG master merge? True