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SRR10502422 Sample ID 265
| Paper identifier | Birkenheuer2020rna |
| SRP accession | SRP193891 |
| GEO accession | GSE130342 |
| Samples | SRR10502422 |
| QC score | 3 |
| Replicate | 2 |
| Protocol | PRO-seq |
| Library prep | ligation |
| Spike-in | D. melanogaster |
| Single/Paired end | single |
| Raw strandedness | reverse |
| Reverse complemented? | True |
| Mapped strandedness | forward |
| Timecourse | False |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | 48 Mb |
| Stranded gene read counts | TXT file | 1 Mb |
| Unstranded bidirectional read counts | TXT file | 21 Mb |
| Tfit bidirectional regions | BED file | 2 Mb |
| dREG bidirectional regions | BED file | 915 Kb |
| All data files | ZIP archive | |
| Organism | H. sapiens |
| Sample type | cell line |
| Cell type | HEp2 |
| Clone/Individual | |
| Strain | |
| Genotype | |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | HSV-1 (MOI 5) | -6 hr | -5 hr | 1 hr |
| treatment | flavopiridol (450 nM) | -6 hr | 0 hr | 6 hr |
| Pipeline info and software versions | |
| Sample notes | length of flavopiridol treatment unclear - could be 1 hr or 6 hr |
| Analysis notes |
| Sample QC score | 3 |
| Sample NRO score | 5 |
| Raw read length | 35 |
| Read depth after trimming | 27,752,690 |
| Duplication proportion | 0.75783 |
| Proportion reads mapped | 0.6909 |
| Exon/intron ratio | 13.6377 |
| Unique read proportion | 0.29042 |
| Total # Tfit bidirectionals | 26329 |
| # Promoter Tfit bidirectionals | 9887 |
| # Intronic Tfit bidirectionals | 11551 |
| # Intergenic Tfit bidirectionals | 5118 |
| Total # dREG bidirectionals | 21043 |
| # Promoter dREG bidirectionals | 8847 |
| # Intronic dREG bidirectionals | 8764 |
| # Intergenic dREG bidirectionals | 3540 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |