DBNascent | Samples

SRR10582184 Sample ID 718

**Data sources**
Paper identifier	Fan2020drb
SRP accession	SRP234556
GEO accession	GSE141373
Samples	SRR10582184

**Technical details**
QC score	1
Replicate	1
Protocol	TT-seq
Library prep	random primed
Spike-in	D. melanogaster
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	124 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	926 Kb
dREG bidirectional regions	BED file	363 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MV4-11
Clone/Individual
Strain
Genotype	Cdk12 F813G/-; Cdk13 F791G/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	4-thiouridine (1 mM)	-5 min	0 min	5 min
vehicle	DMSO	-25 min	-5 min	20 min

**Extended notes**
Pipeline info and software versions
Sample notes	4sU-seq; Mutants are sensitive to 1-NM-PP1
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	1
Raw read length	75
Read depth after trimming	32,827,767
Duplication proportion	0.2421
Proportion reads mapped	0.8545
Exon/intron ratio	2.54047
Unique read proportion	0.86535

**Bidirectionals**
Total # Tfit bidirectionals	10547
# Promoter Tfit bidirectionals	4233
# Intronic Tfit bidirectionals	4365
# Intergenic Tfit bidirectionals	970
Total # dREG bidirectionals	8374
# Promoter dREG bidirectionals	3615
# Intronic dREG bidirectionals	2953
# Intergenic dREG bidirectionals	1130
Part of Tfit master merge?	False
Part of dREG master merge?	False