SRR10582185 Sample ID 719

Data sources
Paper identifier Fan2020drb
SRP accession SRP234556
GEO accession GSE141373
Samples SRR10582185
Technical details
QC score 1
Replicate 1
Protocol TT-seq
Library prep random primed
Spike-in D. melanogaster
Single/Paired end single
Raw strandedness reverse
Reverse complemented? True
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type MV4-11
Clone/Individual
Strain
Genotype Cdk12 F813G/-; Cdk13 F791G/-
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment 1-NM-PP1 (10 microM) -25 min -5 min 20 min
treatment 4-thiouridine (1 mM) -5 min 0 min 5 min
Extended notes
Pipeline info and software versions
Sample notes 4sU-seq; Mutants are sensitive to 1-NM-PP1
Analysis notes
Sample metrics
Sample QC score 1
Sample NRO score 4
Raw read length 75
Read depth after trimming 33,370,927
Duplication proportion 0.38833
Proportion reads mapped 0.6696
Exon/intron ratio 8.52401
Unique read proportion 0.70335
Bidirectionals
Total # Tfit bidirectionals 29012
# Promoter Tfit bidirectionals 9171
# Intronic Tfit bidirectionals 13110
# Intergenic Tfit bidirectionals 3550
Total # dREG bidirectionals 14342
# Promoter dREG bidirectionals 5569
# Intronic dREG bidirectionals 5307
# Intergenic dREG bidirectionals 2189
Part of Tfit master merge? False
Part of dREG master merge? False