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SRR10582187 Sample ID 721
| Paper identifier | Fan2020drb |
| SRP accession | SRP234556 |
| GEO accession | GSE141373 |
| Samples | SRR10582187 |
| QC score | 1 |
| Replicate | 1 |
| Protocol | TT-seq |
| Library prep | random primed |
| Spike-in | D. melanogaster |
| Single/Paired end | single |
| Raw strandedness | reverse |
| Reverse complemented? | True |
| Mapped strandedness | forward |
| Timecourse | False |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | |
| Stranded gene read counts | TXT file | |
| Unstranded bidirectional read counts | TXT file | |
| Tfit bidirectional regions | BED file | |
| dREG bidirectional regions | BED file | |
| All data files | ZIP archive | |
| Organism | H. sapiens |
| Sample type | cell line |
| Cell type | MV4-11 |
| Clone/Individual | |
| Strain | |
| Genotype | Cdk12 F813G/-; Cdk13 F791G/F791G |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | 1-NM-PP1 (10 microM) | -25 min | -5 min | 20 min |
| treatment | 4-thiouridine (1 mM) | -5 min | 0 min | 5 min |
| Pipeline info and software versions | |
| Sample notes | 4sU-seq; Mutants are sensitive to 1-NM-PP1 |
| Analysis notes |
| Sample QC score | 1 |
| Sample NRO score | 4 |
| Raw read length | 75 |
| Read depth after trimming | 32,292,333 |
| Duplication proportion | 0.40439 |
| Proportion reads mapped | 0.7271 |
| Exon/intron ratio | 8.93198 |
| Unique read proportion | 0.69747 |
| Total # Tfit bidirectionals | 28303 |
| # Promoter Tfit bidirectionals | 8820 |
| # Intronic Tfit bidirectionals | 12729 |
| # Intergenic Tfit bidirectionals | 3351 |
| Total # dREG bidirectionals | 14271 |
| # Promoter dREG bidirectionals | 5534 |
| # Intronic dREG bidirectionals | 5239 |
| # Intergenic dREG bidirectionals | 2062 |
| Part of Tfit master merge? | False |
| Part of dREG master merge? | False |