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SRR10582257 Sample ID 748
| Paper identifier | Fan2020drb |
| SRP accession | SRP234556 |
| GEO accession | GSE141373 |
| Samples | SRR10582257 |
| QC score | 1 |
| Replicate | 1 |
| Protocol | PRO-seq |
| Library prep | random primed |
| Spike-in | None |
| Single/Paired end | single |
| Raw strandedness | reverse |
| Reverse complemented? | True |
| Mapped strandedness | forward |
| Timecourse | False |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | |
| Stranded gene read counts | TXT file | |
| Unstranded bidirectional read counts | TXT file | |
| Tfit bidirectional regions | BED file | |
| dREG bidirectional regions | BED file | |
| All data files | ZIP archive | |
| Organism | H. sapiens |
| Sample type | cell line |
| Cell type | MV4-11 |
| Clone/Individual | |
| Strain | |
| Genotype | Cdk12 F813G/-; Cdk13 F791G/- |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | 1-NM-PP1 (10 microM) | -285 min | -45 min | 4 hr |
| treatment | DRB (100 microM) | -285 min | -45 min | 4 hr |
| Pipeline info and software versions | |
| Sample notes | Mutants are sensitive to 1-NM-PP1; Sample title doesn't match mutant metadata - used title info |
| Analysis notes |
| Sample QC score | 1 |
| Sample NRO score | 2 |
| Raw read length | 75 |
| Read depth after trimming | 23,882,264 |
| Duplication proportion | 0.37705 |
| Proportion reads mapped | 0.9386 |
| Exon/intron ratio | 4.56332 |
| Unique read proportion | 0.72665 |
| Total # Tfit bidirectionals | 37906 |
| # Promoter Tfit bidirectionals | 10102 |
| # Intronic Tfit bidirectionals | 17434 |
| # Intergenic Tfit bidirectionals | 7420 |
| Total # dREG bidirectionals | 18814 |
| # Promoter dREG bidirectionals | 5467 |
| # Intronic dREG bidirectionals | 8231 |
| # Intergenic dREG bidirectionals | 4099 |
| Part of Tfit master merge? | False |
| Part of dREG master merge? | False |