DBNascent | Samples

SRR10582257 Sample ID 748

**Data sources**
Paper identifier	Fan2020drb
SRP accession	SRP234556
GEO accession	GSE141373
Samples	SRR10582257

**Technical details**
QC score	1
Replicate	1
Protocol	PRO-seq
Library prep	random primed
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	93 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	820 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MV4-11
Clone/Individual
Strain
Genotype	Cdk12 F813G/-; Cdk13 F791G/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	1-NM-PP1 (10 microM)	-285 min	-45 min	4 hr
treatment	DRB (100 microM)	-285 min	-45 min	4 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Mutants are sensitive to 1-NM-PP1; Sample title doesn't match mutant metadata - used title info
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	75
Read depth after trimming	23,882,264
Duplication proportion	0.37705
Proportion reads mapped	0.9386
Exon/intron ratio	4.56332
Unique read proportion	0.72665

**Bidirectionals**
Total # Tfit bidirectionals	37906
# Promoter Tfit bidirectionals	10102
# Intronic Tfit bidirectionals	17434
# Intergenic Tfit bidirectionals	7420
Total # dREG bidirectionals	18814
# Promoter dREG bidirectionals	5467
# Intronic dREG bidirectionals	8231
# Intergenic dREG bidirectionals	4099
Part of Tfit master merge?	False
Part of dREG master merge?	False