DBNascent | Samples

SRR10582260 Sample ID 751

**Data sources**
Paper identifier	Fan2020drb
SRP accession	SRP234556
GEO accession	GSE141373
Samples	SRR10582260

**Technical details**
QC score	1
Replicate	1
Protocol	PRO-seq
Library prep	random primed
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	92 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	296 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MV4-11
Clone/Individual
Strain
Genotype	Cdk12 F813G/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	1-NM-PP1 (10 microM)	-285 min	-45 min	4 hr
treatment	DRB (100 microM)	-285 min	-45 min	4 hr

**Extended notes**
Pipeline info and software versions
Sample notes	4sU-seq; Mutants are sensitive to 1-NM-PP1; Labeled "input" - no indication of what that means, treat as a separate experiment, not a replicate
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	3
Raw read length	75
Read depth after trimming	23,461,518
Duplication proportion	0.32283
Proportion reads mapped	0.9283
Exon/intron ratio	5.27723
Unique read proportion	0.75171

**Bidirectionals**
Total # Tfit bidirectionals	11959
# Promoter Tfit bidirectionals	2868
# Intronic Tfit bidirectionals	5718
# Intergenic Tfit bidirectionals	1005
Total # dREG bidirectionals	6860
# Promoter dREG bidirectionals	2365
# Intronic dREG bidirectionals	2752
# Intergenic dREG bidirectionals	850
Part of Tfit master merge?	False
Part of dREG master merge?	False