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SRR10582260 Sample ID 751
| Paper identifier | Fan2020drb |
| SRP accession | SRP234556 |
| GEO accession | GSE141373 |
| Samples | SRR10582260 |
| QC score | 1 |
| Replicate | 1 |
| Protocol | PRO-seq |
| Library prep | random primed |
| Spike-in | None |
| Single/Paired end | single |
| Raw strandedness | reverse |
| Reverse complemented? | True |
| Mapped strandedness | forward |
| Timecourse | False |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | |
| Stranded gene read counts | TXT file | |
| Unstranded bidirectional read counts | TXT file | |
| Tfit bidirectional regions | BED file | |
| dREG bidirectional regions | BED file | |
| All data files | ZIP archive | |
| Organism | H. sapiens |
| Sample type | cell line |
| Cell type | MV4-11 |
| Clone/Individual | |
| Strain | |
| Genotype | Cdk12 F813G/- |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | 1-NM-PP1 (10 microM) | -285 min | -45 min | 4 hr |
| treatment | DRB (100 microM) | -285 min | -45 min | 4 hr |
| Pipeline info and software versions | |
| Sample notes | 4sU-seq; Mutants are sensitive to 1-NM-PP1; Labeled "input" - no indication of what that means, treat as a separate experiment, not a replicate |
| Analysis notes |
| Sample QC score | 1 |
| Sample NRO score | 3 |
| Raw read length | 75 |
| Read depth after trimming | 23,461,518 |
| Duplication proportion | 0.32283 |
| Proportion reads mapped | 0.9283 |
| Exon/intron ratio | 5.27723 |
| Unique read proportion | 0.75171 |
| Total # Tfit bidirectionals | 11959 |
| # Promoter Tfit bidirectionals | 2868 |
| # Intronic Tfit bidirectionals | 5718 |
| # Intergenic Tfit bidirectionals | 1005 |
| Total # dREG bidirectionals | 6860 |
| # Promoter dREG bidirectionals | 2365 |
| # Intronic dREG bidirectionals | 2752 |
| # Intergenic dREG bidirectionals | 850 |
| Part of Tfit master merge? | False |
| Part of dREG master merge? | False |