DBNascent | Samples

SRR10669540 Sample ID 2312

**Data sources**
Paper identifier	Smith2021peppro
SRP accession	SRP236879
GEO accession	GSE141843
Samples	SRR10669540

**Technical details**
QC score	1
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	146 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	964 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	ESC
Clone/Individual	H9
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	romidepsin (200 nM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	No library details specified, cites Sathyan2019
Analysis notes	Ran qc redo nextflow; Previous identifier until 6/15/21 was Guertin2019unpublished, some documentation reflects that

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	35
Read depth after trimming	26,049,630
Duplication proportion	0.14092
Proportion reads mapped	0.6707
Exon/intron ratio	4.56427
Unique read proportion	0.78063

**Bidirectionals**
Total # Tfit bidirectionals	31731
# Promoter Tfit bidirectionals	16014
# Intronic Tfit bidirectionals	11819
# Intergenic Tfit bidirectionals	4969
Total # dREG bidirectionals	22123
# Promoter dREG bidirectionals	9442
# Intronic dREG bidirectionals	9029
# Intergenic dREG bidirectionals	3505
Part of Tfit master merge?	True
Part of dREG master merge?	True