DBNascent | Samples

SRR10915526 Sample ID 197

**Data sources**
Paper identifier	Barbieri2020rapid
SRP accession	SRP242477
GEO accession	GSE143844
Samples	SRR10915526

**Technical details**
QC score	5
Replicate	1
Protocol	GRO-seq variant
Library prep	random primed
Spike-in	D. melanogaster
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	14 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HeLa
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	EGF (100 ng/mL)	-15 min	0 min	15 min

**Extended notes**
Pipeline info and software versions
Sample notes	fastGRO (run-on with 4sU); 2.5 million cells, biotin-HPDP for biotinylation
Analysis notes	Unusable due to extreme number of duplicates leading to sparse coverage

**Sample metrics**
Sample QC score	5
Sample NRO score	2
Raw read length	35
Read depth after trimming	57,534,480
Duplication proportion	0.96337
Proportion reads mapped	0.7438
Exon/intron ratio	4.47009
Unique read proportion	0.02337

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	True
Part of dREG master merge?	True