DBNascent | Samples

SRR11024628 Sample ID 159

**Data sources**
Paper identifier	Aoi2020nelf
SRP accession	SRP247346
GEO accession	GSE144786
Samples	SRR11024628

**Technical details**
QC score	4
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	D. melanogaster
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	54 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	DLD1 Flp-In
Clone/Individual
Strain
Genotype	Tir1 (Flp knock-in); Nelf-C auxin-inducible degron
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	auxin (500 microM)	-3 hr	-1 hr	2 hr
treatment	heat shock (42 Celsius)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	treatment conditions not well-defined - mostly in figure legends, but still ambiguous
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	3
Raw read length	56
Read depth after trimming	46,000,579
Duplication proportion	0.85144
Proportion reads mapped	0.7741
Exon/intron ratio	5.61452
Unique read proportion	0.36046

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False