DBNascent | Samples

SRR11273559 Sample ID 1038

**Data sources**
Paper identifier	Jaeger2020selective
SRP accession	SRP227191
GEO accession	GSE139463
Samples	SRR11273559

**Technical details**
QC score	1
Replicate	2
Protocol	TT-seq
Library prep	random primed
Spike-in	SIRV Set 3
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	447 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HCT116
Clone/Individual
Strain
Genotype	Med14 dTAG-inducible degron
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	4-thiouridine (500 microM)	-5 min	0 min	5 min
vehicle	DMSO	-2 hr	0 hr	2 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Labeled as experiment MJ-20-5, mentions to only cross-compare within experiments
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	1
Raw read length	35
Read depth after trimming	78,477,742
Duplication proportion	0.22248
Proportion reads mapped	0.9537
Exon/intron ratio	2.05825
Unique read proportion	0.81986

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	True
Part of dREG master merge?	True