DBNascent | Samples

SRR11785051 Sample ID 1716

**Data sources**
Paper identifier	Liu2021transcription
SRP accession	SRP261462
GEO accession	GSE150491
Samples	SRR11785051

**Technical details**
QC score	1
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	D. melanogaster
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	193 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	874 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	primary cell
Cell type	CD34+ erythroblast
Clone/Individual	sgRNA Bcl11a exon 2
Strain
Genotype	Bcl11a -/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	sgRNAs were CRISPRed into genome 72 hours before harvest
Analysis notes	Preseq required separate run with -D flag; Previously in DB until 4/19/2021 as Liu2020unpublished - all scripts have been changed, but previous outerr files and logs reflect previous id

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	41
Read depth after trimming	47,868,057
Duplication proportion	0.34881
Proportion reads mapped	0.7199
Exon/intron ratio	4.56534
Unique read proportion	0.70534

**Bidirectionals**
Total # Tfit bidirectionals	24282
# Promoter Tfit bidirectionals	13480
# Intronic Tfit bidirectionals	8681
# Intergenic Tfit bidirectionals	3056
Total # dREG bidirectionals	20059
# Promoter dREG bidirectionals	7767
# Intronic dREG bidirectionals	8335
# Intergenic dREG bidirectionals	3384
Part of Tfit master merge?	True
Part of dREG master merge?	True