SRR12634527 Sample ID 206

Data sources
Paper identifier Barbieri2020rapid
SRP accession SRP242477
GEO accession GSE143844
Samples SRR12634527
Technical details
QC score 5
Replicate 1
Protocol GRO-seq variant
Library prep random primed
Spike-in D. melanogaster
Single/Paired end paired
Raw strandedness read1 reverse
Reverse complemented? False
Mapped strandedness read1 reverse
Timecourse False
Control/Experimental control
Wildtype & Untreated? True
Outlier No
Unusable No
Downloads
TDF visualization file TDF file 20 Mb
Stranded gene read counts TXT file 1 Mb
Unstranded bidirectional read counts TXT file 21 Mb
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type iPSC
Clone/Individual SV20
Strain
Genotype
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
no treatment None None None
Extended notes
Pipeline info and software versions
Sample notes fastGRO (run-on with 4sU); 5 million cells, biotin-MTS for biotinylation
Analysis notes
Sample metrics
Sample QC score 5
Sample NRO score 3
Raw read length 35
Read depth after trimming 35,347,875
Duplication proportion 0.8475
Proportion reads mapped 0.9634
Exon/intron ratio 5.81784
Unique read proportion 0.04691
Bidirectionals
Total # Tfit bidirectionals None
# Promoter Tfit bidirectionals None
# Intronic Tfit bidirectionals None
# Intergenic Tfit bidirectionals None
Total # dREG bidirectionals None
# Promoter dREG bidirectionals None
# Intronic dREG bidirectionals None
# Intergenic dREG bidirectionals None
Part of Tfit master merge? True
Part of dREG master merge? True