DBNascent | Samples

SRR12699342 Sample ID 2236

**Data sources**
Paper identifier	Schick2021acute
SRP accession	SRP255443
GEO accession	GSE148175
Samples	SRR12699342

**Technical details**
QC score	3
Replicate	1
Protocol	PRO-seq
Library prep	not noted in GEO or publication
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	78 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	785 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HAP1
Clone/Individual
Strain
Genotype	Smarca4 -/-; Smarca2 dTAG-inducible degron
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	dTAG47 (300 nM)	-3 hr	0 hr	3 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Cites Jaeger2020selective, so might be random primed library, and might have Drosophila spike-ins
Analysis notes	Previously in DB until 4/19/2021 as Schick2020unpublished - scripts have been changed, but all previous outerr and log files reflect previous id

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	13
Read depth after trimming	40,080,484
Duplication proportion	0.71505
Proportion reads mapped	0.9812
Exon/intron ratio	2.70253
Unique read proportion	0.58416

**Bidirectionals**
Total # Tfit bidirectionals	30006
# Promoter Tfit bidirectionals	8920
# Intronic Tfit bidirectionals	15606
# Intergenic Tfit bidirectionals	3777
Total # dREG bidirectionals	18225
# Promoter dREG bidirectionals	7601
# Intronic dREG bidirectionals	7635
# Intergenic dREG bidirectionals	2222
Part of Tfit master merge?	True
Part of dREG master merge?	True