DBNascent | Samples

SRR13114375 Sample ID 2815

**Data sources**
Paper identifier	Zhang2021physiological
SRP accession	SRP293909
GEO accession	GSE162010
Samples	SRR13114375

**Technical details**
QC score	4
Replicate	3
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	27 Mb
Stranded gene read counts	TXT file	913 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	618 Kb
dREG bidirectional regions	BED file	182 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain
Genotype	Aid -/-; 3' IgH CBE -/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Originally in db until 4/19/2021 as Zhang2021unpublished, all scripts changed but original outerr and logs reflect previous ID

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	151
Read depth after trimming	19,597,649
Duplication proportion	0.87801
Proportion reads mapped	0.7835
Exon/intron ratio	1.71886
Unique read proportion	0.16414

**Bidirectionals**
Total # Tfit bidirectionals	7022
# Promoter Tfit bidirectionals	1912
# Intronic Tfit bidirectionals	3103
# Intergenic Tfit bidirectionals	1024
Total # dREG bidirectionals	4206
# Promoter dREG bidirectionals	1423
# Intronic dREG bidirectionals	1589
# Intergenic dREG bidirectionals	804
Part of Tfit master merge?	True
Part of dREG master merge?	True