DBNascent | Samples

SRR13632939 Sample ID 892

**Data sources**
Paper identifier	Gibson2016chemical
SRP accession	SRP064970
GEO accession	GSE74142
Samples	SRR13632939

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	80 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	816 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype	shRNA Parp1 (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol	-40 min	0 min	40 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	1
Raw read length	43
Read depth after trimming	19,366,888
Duplication proportion	0.33879
Proportion reads mapped	0.8684
Exon/intron ratio	2.07849
Unique read proportion	0.71681

**Bidirectionals**
Total # Tfit bidirectionals	30165
# Promoter Tfit bidirectionals	10393
# Intronic Tfit bidirectionals	13209
# Intergenic Tfit bidirectionals	5906
Total # dREG bidirectionals	18899
# Promoter dREG bidirectionals	8293
# Intronic dREG bidirectionals	6971
# Intergenic dREG bidirectionals	3956
Part of Tfit master merge?	True
Part of dREG master merge?	True