SRR1519033 Sample ID 824

Data sources
Paper identifier Franco2015tnfalpha
SRP accession SRP044608
GEO accession GSE59531
Samples SRR1519033
Technical details
QC score 1
Replicate 2
Protocol GRO-seq
Library prep circularization
Spike-in None
Single/Paired end single
Raw strandedness forward
Reverse complemented? False
Mapped strandedness forward
Timecourse False
Control/Experimental control
Wildtype & Untreated? True
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type MCF7
Clone/Individual
Strain
Genotype
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
vehicle ethanol -40 min 0 min 40 min
Extended notes
Pipeline info and software versions
Sample notes
Analysis notes Requires "qin=33" flag for bbduk - issue with recognizing phred encoding; Ran qc redo nextflow
Sample metrics
Sample QC score 1
Sample NRO score 3
Raw read length 50
Read depth after trimming 20,926,011
Duplication proportion 0.41424
Proportion reads mapped 0.8706
Exon/intron ratio 5.54038
Unique read proportion 0.63068
Bidirectionals
Total # Tfit bidirectionals 21396
# Promoter Tfit bidirectionals 1774
# Intronic Tfit bidirectionals 14586
# Intergenic Tfit bidirectionals 2205
Total # dREG bidirectionals 21176
# Promoter dREG bidirectionals 1334
# Intronic dREG bidirectionals 14727
# Intergenic dREG bidirectionals 2499
Part of Tfit master merge? True
Part of dREG master merge? True