SRR1519034 Sample ID 825

Data sources
Paper identifier Franco2015tnfalpha
SRP accession SRP044608
GEO accession GSE59531
Samples SRR1519034
Technical details
QC score 1
Replicate 3
Protocol GRO-seq
Library prep circularization
Spike-in None
Single/Paired end single
Raw strandedness forward
Reverse complemented? False
Mapped strandedness forward
Timecourse False
Control/Experimental control
Wildtype & Untreated? True
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type MCF7
Clone/Individual
Strain
Genotype
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
vehicle ethanol -40 min 0 min 40 min
Extended notes
Pipeline info and software versions
Sample notes
Analysis notes Requires "qin=33" flag for bbduk - issue with recognizing phred encoding; Ran qc redo nextflow
Sample metrics
Sample QC score 1
Sample NRO score 3
Raw read length 50
Read depth after trimming 26,977,087
Duplication proportion 0.36028
Proportion reads mapped 0.8915
Exon/intron ratio 2.89615
Unique read proportion 0.73166
Bidirectionals
Total # Tfit bidirectionals 25785
# Promoter Tfit bidirectionals 2261
# Intronic Tfit bidirectionals 18051
# Intergenic Tfit bidirectionals 3021
Total # dREG bidirectionals 29537
# Promoter dREG bidirectionals 1781
# Intronic dREG bidirectionals 21583
# Intergenic dREG bidirectionals 3852
Part of Tfit master merge? True
Part of dREG master merge? True