SRR1519041 Sample ID 832

Data sources
Paper identifier Franco2015tnfalpha
SRP accession SRP044608
GEO accession GSE59531
Samples SRR1519041
Technical details
QC score 1
Replicate 1
Protocol GRO-seq
Library prep circularization
Spike-in None
Single/Paired end single
Raw strandedness forward
Reverse complemented? False
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type MCF7
Clone/Individual
Strain
Genotype
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment 17-beta-estradiol (100 nM) -40 min 0 min 40 min
treatment TNF-alpha (25 ng/mL) -40 min 0 min 40 min
Extended notes
Pipeline info and software versions
Sample notes
Analysis notes Requires "qin=33" flag for bbduk - issue with recognizing phred encoding; Ran qc redo nextflow
Sample metrics
Sample QC score 1
Sample NRO score 3
Raw read length 50
Read depth after trimming 21,889,080
Duplication proportion 0.41082
Proportion reads mapped 0.8524
Exon/intron ratio 3.67394
Unique read proportion 0.63848
Bidirectionals
Total # Tfit bidirectionals 22993
# Promoter Tfit bidirectionals 1729
# Intronic Tfit bidirectionals 16279
# Intergenic Tfit bidirectionals 2491
Total # dREG bidirectionals 23390
# Promoter dREG bidirectionals 1318
# Intronic dREG bidirectionals 17148
# Intergenic dREG bidirectionals 2857
Part of Tfit master merge? True
Part of dREG master merge? True