DBNascent | Samples

SRR1542323 Sample ID 1614

**Data sources**
Paper identifier	Liu2014enhancer
SRP accession	SRP045391
GEO accession	GSE60271
Samples	SRR1542323

**Technical details**
QC score	5
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	22 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	489 Kb
dREG bidirectional regions	BED file	179 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct	siRNA Gata3

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol (100nM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	5
Sample NRO score	3
Raw read length	50
Read depth after trimming	41,267,346
Duplication proportion	0.96108
Proportion reads mapped	0.895
Exon/intron ratio	5.73404
Unique read proportion	0.09535

**Bidirectionals**
Total # Tfit bidirectionals	5570
# Promoter Tfit bidirectionals	2865
# Intronic Tfit bidirectionals	1820
# Intergenic Tfit bidirectionals	312
Total # dREG bidirectionals	4176
# Promoter dREG bidirectionals	2753
# Intronic dREG bidirectionals	1032
# Intergenic dREG bidirectionals	399
Part of Tfit master merge?	True
Part of dREG master merge?	True