DBNascent | Samples

SRR1542327 Sample ID 1618

**Data sources**
Paper identifier	Liu2014enhancer
SRP accession	SRP045391
GEO accession	GSE60271
Samples	SRR1542327

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	119 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	902 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct	shRNA RAR-alpha/RAR-gamma

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
vehicle	DMSO	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Vehicle may have been EtOH - not well specified
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	50
Read depth after trimming	48,746,619
Duplication proportion	0.57287
Proportion reads mapped	0.8987
Exon/intron ratio	3.52294
Unique read proportion	0.46709

**Bidirectionals**
Total # Tfit bidirectionals	25300
# Promoter Tfit bidirectionals	11778
# Intronic Tfit bidirectionals	9845
# Intergenic Tfit bidirectionals	3932
Total # dREG bidirectionals	20736
# Promoter dREG bidirectionals	8594
# Intronic dREG bidirectionals	8565
# Intergenic dREG bidirectionals	3668
Part of Tfit master merge?	True
Part of dREG master merge?	True