DBNascent | Samples

SRR1542331 Sample ID 1622

**Data sources**
Paper identifier	Liu2014enhancer
SRP accession	SRP045391
GEO accession	GSE60271
Samples	SRR1542331

**Technical details**
QC score	2
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	123 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	866 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct	shRNA control

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol (100nM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	50
Read depth after trimming	45,813,970
Duplication proportion	0.54662
Proportion reads mapped	0.949
Exon/intron ratio	3.6
Unique read proportion	0.4907

**Bidirectionals**
Total # Tfit bidirectionals	23572
# Promoter Tfit bidirectionals	10944
# Intronic Tfit bidirectionals	9037
# Intergenic Tfit bidirectionals	3717
Total # dREG bidirectionals	19955
# Promoter dREG bidirectionals	8545
# Intronic dREG bidirectionals	8085
# Intergenic dREG bidirectionals	3390
Part of Tfit master merge?	True
Part of dREG master merge?	True