DBNascent | Samples

SRR1611835 Sample ID 1831

**Data sources**
Paper identifier	Meng2014convergent
SRP accession	SRP048895
GEO accession	GSE62296
Samples	SRR1611835

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	273 Mb
Stranded gene read counts	TXT file	932 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	2 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain
Genotype	Vhb18 heavy chain knock-in
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	anti-CD40 (500 ng/mL)	-60 hr	0 hr	60 hr
treatment	interleukin-4 (25 ng/mL)	-60 hr	0 hr	60 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	51
Read depth after trimming	178,627,917
Duplication proportion	0.72479
Proportion reads mapped	0.803
Exon/intron ratio	2.59426
Unique read proportion	0.64419

**Bidirectionals**
Total # Tfit bidirectionals	28158
# Promoter Tfit bidirectionals	8666
# Intronic Tfit bidirectionals	10805
# Intergenic Tfit bidirectionals	6228
Total # dREG bidirectionals	37986
# Promoter dREG bidirectionals	7410
# Intronic dREG bidirectionals	16532
# Intergenic dREG bidirectionals	11252
Part of Tfit master merge?	True
Part of dREG master merge?	True