DBNascent | Samples

SRR1611836 Sample ID 1832

**Data sources**
Paper identifier	Meng2014convergent
SRP accession	SRP048895
GEO accession	GSE62296
Samples	SRR1611836

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	181 Mb
Stranded gene read counts	TXT file	936 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain
Genotype	Vhb18 heavy chain knock-in
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	anti-CD40 (500 ng/mL)	-60 hr	0 hr	60 hr
treatment	interleukin-4 (25 ng/mL)	-60 hr	0 hr	60 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	51
Read depth after trimming	202,327,151
Duplication proportion	0.87715
Proportion reads mapped	0.8816
Exon/intron ratio	2.31403
Unique read proportion	0.60542

**Bidirectionals**
Total # Tfit bidirectionals	26758
# Promoter Tfit bidirectionals	6513
# Intronic Tfit bidirectionals	11878
# Intergenic Tfit bidirectionals	5960
Total # dREG bidirectionals	32985
# Promoter dREG bidirectionals	7577
# Intronic dREG bidirectionals	14169
# Intergenic dREG bidirectionals	8550
Part of Tfit master merge?	True
Part of dREG master merge?	True