DBNascent | Samples

SRR1652072 Sample ID 2607

**Data sources**
Paper identifier	Wang2015lsd1n
SRP accession	SRP049780
GEO accession	GSE63271
Samples	SRR1652072

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	42 Mb
Stranded gene read counts	TXT file	911 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	557 Kb
dREG bidirectional regions	BED file	190 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	cortical neuron
Clone/Individual
Strain
Genotype	Lsd1 exon 8a fl/fl; Nestin-Cre
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	KCl (55 mM)	-1 hr	0 hr	1 hr
treatment	tetrodotoxin (1 microM)	-15 hr	-1 hr	14 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Labeled as hs82l1, but unclear the meaning
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	50
Read depth after trimming	35,005,948
Duplication proportion	0.74399
Proportion reads mapped	0.6956
Exon/intron ratio	4.30246
Unique read proportion	0.27364

**Bidirectionals**
Total # Tfit bidirectionals	6644
# Promoter Tfit bidirectionals	2387
# Intronic Tfit bidirectionals	1942
# Intergenic Tfit bidirectionals	146
Total # dREG bidirectionals	4424
# Promoter dREG bidirectionals	2042
# Intronic dREG bidirectionals	1113
# Intergenic dREG bidirectionals	713
Part of Tfit master merge?	True
Part of dREG master merge?	True