DBNascent | Samples

SRR1652073 Sample ID 2607

**Data sources**
Paper identifier	Wang2015lsd1n
SRP	SRP049780
Samples	SRR1652073

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Timecourse	True
Control/Experimental	experimental
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	41 Mb
Stranded gene read counts	TXT file	911 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	477 Kb
dREG bidirectional regions	BED file	166 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	cortical neuron
Clone/Individual
Strain
Genotype	Lsd1 exon 8a fl/fl; Nestin-Cre
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	KCl (55 mM)	-3 hr	0 hr	3 hr
treatment	tetrodotoxin (1 microM)	-17 hr	-3 hr	14 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Labeled as hs82l1, but unclear the meaning
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	50
Read depth after trimming	31,668,765
Duplication proportion	0.7246
Proportion reads mapped	0.6854
Exon/intron ratio	4.55949
Unique read proportion	0.29505

**Bidirectionals**
Total # Tfit bidirectionals	5706
# Promoter Tfit bidirectionals	1824
# Intronic Tfit bidirectionals	1781
# Intergenic Tfit bidirectionals	80
Total # dREG bidirectionals	3857
# Promoter dREG bidirectionals	1562
# Intronic dREG bidirectionals	1015
# Intergenic dREG bidirectionals	748
Part of Tfit master merge?	True
Part of dREG master merge?	True