DBNascent | Samples

SRR1652075 Sample ID 2609

**Data sources**
Paper identifier	Wang2015lsd1n
SRP	SRP049780
Samples	SRR1652075

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Timecourse	True
Control/Experimental	experimental
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	78 Mb
Stranded gene read counts	TXT file	916 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	495 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	cortical neuron
Clone/Individual
Strain
Genotype	Lsd1 exon 8a fl/fl
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	KCl (55 mM)	-1 hr	0 hr	1 hr
treatment	tetrodotoxin (1 microM)	-15 hr	-1 hr	14 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Labeled as hs43l4, but unclear the meaning - may be replicates?
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	1
Raw read length	50
Read depth after trimming	36,884,621
Duplication proportion	0.56438
Proportion reads mapped	0.7633
Exon/intron ratio	2.58869
Unique read proportion	0.49746

**Bidirectionals**
Total # Tfit bidirectionals	12891
# Promoter Tfit bidirectionals	5940
# Intronic Tfit bidirectionals	4393
# Intergenic Tfit bidirectionals	1076
Total # dREG bidirectionals	11493
# Promoter dREG bidirectionals	5391
# Intronic dREG bidirectionals	3451
# Intergenic dREG bidirectionals	1626
Part of Tfit master merge?	True
Part of dREG master merge?	True