DBNascent | Samples

SRR1661567 Sample ID 796

**Data sources**
Paper identifier	Fong2014pre
SRP	SRP050215
Samples	SRR1661567

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Timecourse	True
Control/Experimental	control
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	20 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	213 Kb
dREG bidirectional regions	BED file	50 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293 Flp-In
Clone/Individual
Strain
Genotype	alpha-amanitin resistant, dox-inducible Rpb1 N792D (Flp knock-in)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	DRB (100 microM)	-200 min	-20 min	3 hr
treatment	alpha-amanitin (2.5 microg/mL)	-2720 min	-200 min	42 hr
treatment	doxycycline (2 microg/mL)	-3560 min	-2720 min	14 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Very dirty data, contamination, many reads thrown out - about half sparse or very sparse; Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	38
Read depth after trimming	6,846,560
Duplication proportion	0.66792
Proportion reads mapped	0.6429
Exon/intron ratio	2.36957
Unique read proportion	0.29575

**Bidirectionals**
Total # Tfit bidirectionals	2407
# Promoter Tfit bidirectionals	1291
# Intronic Tfit bidirectionals	969
# Intergenic Tfit bidirectionals	205
Total # dREG bidirectionals	1151
# Promoter dREG bidirectionals	605
# Intronic dREG bidirectionals	275
# Intergenic dREG bidirectionals	305
Part of Tfit master merge?	True
Part of dREG master merge?	True