DBNascent | Samples

SRR1661573 Sample ID 802

**Data sources**
Paper identifier	Fong2014pre
SRP	SRP050215
Samples	SRR1661573

**Technical details**
QC score	5
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Timecourse	True
Control/Experimental	experimental
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	11 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	42 Kb
dREG bidirectional regions	BED file	15 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293 Flp-In
Clone/Individual
Strain
Genotype	alpha-amanitin resistant, dox-inducible Rpb1 N792D/H1108Y (Flp knock-in)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	DRB (100 microM)	-3 hr	0 hr	3 hr
treatment	alpha-amanitin (2.5 microg/mL)	-45 hr	-3 hr	42 hr
treatment	doxycycline (2 microg/mL)	-59 hr	-45 hr	14 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Labeled H1106Y mutant in SRA/GEO, H1108Y in paper and H1108 matches RefSeq
Analysis notes	Very dirty data, contamination, many reads thrown out - about half sparse or very sparse; Ran qc redo nextflow

**Sample metrics**
Sample QC score	5
Sample NRO score	1
Raw read length	40
Read depth after trimming	4,125,732
Duplication proportion	0.83004
Proportion reads mapped	0.5761
Exon/intron ratio	2.22222
Unique read proportion	0.12415

**Bidirectionals**
Total # Tfit bidirectionals	456
# Promoter Tfit bidirectionals	299
# Intronic Tfit bidirectionals	170
# Intergenic Tfit bidirectionals	48
Total # dREG bidirectionals	341
# Promoter dREG bidirectionals	130
# Intronic dREG bidirectionals	79
# Intergenic dREG bidirectionals	146
Part of Tfit master merge?	True
Part of dREG master merge?	True