DBNascent | Samples

SRR1661578 Sample ID 807

**Data sources**
Paper identifier	Fong2014pre
SRP accession	SRP050215
GEO accession	GSE63375
Samples	SRR1661578

**Technical details**
QC score	5
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	12 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	48 Kb
dREG bidirectional regions	BED file	20 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293 Flp-In
Clone/Individual
Strain
Genotype	alpha-amanitin resistant, dox-inducible Rpb1 N792D/E1126G (Flp knock-in)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	DRB (100 microM)	-190 min	-10 min	3 hr
treatment	alpha-amanitin (2.5 microg/mL)	-2710 min	-190 min	42 hr
treatment	doxycycline (2 microg/mL)	-3550 min	-2710 min	14 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Very dirty data, contamination, many reads thrown out - about half sparse or very sparse; Ran qc redo nextflow

**Sample metrics**
Sample QC score	5
Sample NRO score	1
Raw read length	40
Read depth after trimming	5,756,620
Duplication proportion	0.72425
Proportion reads mapped	0.4165
Exon/intron ratio	2.55814
Unique read proportion	0.21817

**Bidirectionals**
Total # Tfit bidirectionals	529
# Promoter Tfit bidirectionals	270
# Intronic Tfit bidirectionals	233
# Intergenic Tfit bidirectionals	66
Total # dREG bidirectionals	454
# Promoter dREG bidirectionals	181
# Intronic dREG bidirectionals	124
# Intergenic dREG bidirectionals	175
Part of Tfit master merge?	True
Part of dREG master merge?	True