SRR1736060 Sample ID 1976

Data sources
Paper identifier Nojima2015mammalian
SRP accession SRP045447
GEO accession GSE60358
Samples SRR1736060
Technical details
QC score 2
Replicate 1
Protocol NET-seq
Library prep ligation
Spike-in None
Single/Paired end paired
Raw strandedness read1 forward
Reverse complemented? True
Mapped strandedness read1 forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type HeLa
Clone/Individual
Strain
Genotype
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment pladienolides B (1 mM) -4 hr 0 hr 4 hr
Extended notes
Pipeline info and software versions
Sample notes mNET-seq with CMA603 PolII antibody
Analysis notes Ran qc redo nextflow
Sample metrics
Sample QC score 2
Sample NRO score 4
Raw read length 51
Read depth after trimming 35,370,346
Duplication proportion 0.62539
Proportion reads mapped 0.9274
Exon/intron ratio 7.37878
Unique read proportion 0.36434
Bidirectionals
Total # Tfit bidirectionals 24111
# Promoter Tfit bidirectionals 10989
# Intronic Tfit bidirectionals 10566
# Intergenic Tfit bidirectionals 2655
Total # dREG bidirectionals 18888
# Promoter dREG bidirectionals 6478
# Intronic dREG bidirectionals 9180
# Intergenic dREG bidirectionals 2722
Part of Tfit master merge? False
Part of dREG master merge? False