DBNascent | Samples

SRR1736060 Sample ID 1976

**Data sources**
Paper identifier	Nojima2015mammalian
SRP accession	SRP045447
GEO accession	GSE60358
Samples	SRR1736060

**Technical details**
QC score	2
Replicate	1
Protocol	NET-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	90 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	819 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HeLa
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	pladienolides B (1 mM)	-4 hr	0 hr	4 hr

**Extended notes**
Pipeline info and software versions
Sample notes	mNET-seq with CMA603 PolII antibody
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	4
Raw read length	51
Read depth after trimming	35,370,346
Duplication proportion	0.62539
Proportion reads mapped	0.9274
Exon/intron ratio	7.37878
Unique read proportion	0.36434

**Bidirectionals**
Total # Tfit bidirectionals	24111
# Promoter Tfit bidirectionals	10989
# Intronic Tfit bidirectionals	10566
# Intergenic Tfit bidirectionals	2655
Total # dREG bidirectionals	18888
# Promoter dREG bidirectionals	6478
# Intronic dREG bidirectionals	9180
# Intergenic dREG bidirectionals	2722
Part of Tfit master merge?	False
Part of dREG master merge?	False