DBNascent | Samples

SRR1736062 Sample ID 1978

**Data sources**
Paper identifier	Nojima2015mammalian
SRP accession	SRP045447
GEO accession	GSE60358
Samples	SRR1736062

**Technical details**
QC score	2
Replicate	1
Protocol	NET-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	110 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	4 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HeLa
Clone/Individual
Strain
Genotype
Construct	siRNA Cpsf73

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	mNET-seq with 8WG16 PolII antibody
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	5
Raw read length	51
Read depth after trimming	35,340,443
Duplication proportion	0.46747
Proportion reads mapped	0.907
Exon/intron ratio	9.5633
Unique read proportion	0.47108

**Bidirectionals**
Total # Tfit bidirectionals	45107
# Promoter Tfit bidirectionals	18621
# Intronic Tfit bidirectionals	17450
# Intergenic Tfit bidirectionals	9387
Total # dREG bidirectionals	27816
# Promoter dREG bidirectionals	8417
# Intronic dREG bidirectionals	11592
# Intergenic dREG bidirectionals	6808
Part of Tfit master merge?	False
Part of dREG master merge?	False