DBNascent | Samples

SRR1772450 Sample ID 1014

**Data sources**
Paper identifier	Illingworth2016polycomb
SRP	SRP052827
Samples	SRR1772450

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Timecourse	False
Control/Experimental	control
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	21 Mb
Stranded gene read counts	TXT file	908 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	909 Kb
dREG bidirectional regions	BED file	226 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual	HV1.5
Strain
Genotype
Construct	Hhex-Venus reporter

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	cell sorting HV-	None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	HV- epiblast-primed cells (Epi-primed); Cells were stained with anti-SSEA1 antibodies and sorted for Venus fluorescence prior to RNA extraction
Analysis notes	Unstranded, a little strange-looking

**Sample metrics**
Sample QC score	4
Sample NRO score	4
Raw read length	42
Read depth after trimming	37,487,819
Duplication proportion	0.9283
Proportion reads mapped	0.5609
Exon/intron ratio	8.08333
Unique read proportion	0.11422

**Bidirectionals**
Total # Tfit bidirectionals	10443
# Promoter Tfit bidirectionals	823
# Intronic Tfit bidirectionals	3825
# Intergenic Tfit bidirectionals	1035
Total # dREG bidirectionals	5237
# Promoter dREG bidirectionals	564
# Intronic dREG bidirectionals	1420
# Intergenic dREG bidirectionals	1515
Part of Tfit master merge?	True
Part of dREG master merge?	True