DBNascent | Samples

SRR1772451 Sample ID 1015

**Data sources**
Paper identifier	Illingworth2016polycomb
SRP accession	SRP052827
GEO accession	GSE65288
Samples	SRR1772451

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	66 Mb
Stranded gene read counts	TXT file	916 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	6 Mb
dREG bidirectional regions	BED file	547 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual	HV1.5
Strain
Genotype
Construct	Hhex-Venus reporter

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	cell sorting HV+	None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	HV+ endoderm-primed cells (PrEn); Cells were stained with anti-SSEA1 antibodies and sorted for Venus fluorescence prior to RNA extraction
Analysis notes	Unstranded, a little strange-looking

**Sample metrics**
Sample QC score	3
Sample NRO score	3
Raw read length	42
Read depth after trimming	48,907,261
Duplication proportion	0.78743
Proportion reads mapped	0.7624
Exon/intron ratio	6.81452
Unique read proportion	0.3615

**Bidirectionals**
Total # Tfit bidirectionals	66342
# Promoter Tfit bidirectionals	1652
# Intronic Tfit bidirectionals	39522
# Intergenic Tfit bidirectionals	5639
Total # dREG bidirectionals	12678
# Promoter dREG bidirectionals	670
# Intronic dREG bidirectionals	4396
# Intergenic dREG bidirectionals	3229
Part of Tfit master merge?	True
Part of dREG master merge?	True