DBNascent | Samples

SRR1772452 Sample ID 1016

**Data sources**
Paper identifier	Illingworth2016polycomb
SRP accession	SRP052827
GEO accession	GSE65288
Samples	SRR1772452

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	25 Mb
Stranded gene read counts	TXT file	910 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	251 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual	HV1.5
Strain
Genotype
Construct	Hhex-Venus reporter

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	cell sorting HV+	None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	HV+ endoderm-primed cells (PrEn); Cells were stained with anti-SSEA1 antibodies and sorted for Venus fluorescence prior to RNA extraction
Analysis notes	Unstranded, a little strange-looking

**Sample metrics**
Sample QC score	4
Sample NRO score	5
Raw read length	42
Read depth after trimming	36,872,238
Duplication proportion	0.90173
Proportion reads mapped	0.5275
Exon/intron ratio	9.3731
Unique read proportion	0.15139

**Bidirectionals**
Total # Tfit bidirectionals	13990
# Promoter Tfit bidirectionals	718
# Intronic Tfit bidirectionals	5776
# Intergenic Tfit bidirectionals	1365
Total # dREG bidirectionals	5817
# Promoter dREG bidirectionals	421
# Intronic dREG bidirectionals	1539
# Intergenic dREG bidirectionals	1825
Part of Tfit master merge?	True
Part of dREG master merge?	True