DBNascent | Samples

SRR1806547 Sample ID 86

**Data sources**
Paper identifier	Andrade2015dna
SRP accession	SRP055100
GEO accession	GSE65985
Samples	SRR1806547

**Technical details**
QC score	1
Replicate	2
Protocol	Bru-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	96 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	305 Kb
dREG bidirectional regions	BED file	168 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HF1
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	bromouridine (2 mM)	-30 min	0 min	30 min

**Extended notes**
Pipeline info and software versions
Sample notes	BrU-seq
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	52
Read depth after trimming	26,119,093
Duplication proportion	0.36508
Proportion reads mapped	0.9689
Exon/intron ratio	2.06034
Unique read proportion	0.70767

**Bidirectionals**
Total # Tfit bidirectionals	3492
# Promoter Tfit bidirectionals	877
# Intronic Tfit bidirectionals	1978
# Intergenic Tfit bidirectionals	284
Total # dREG bidirectionals	3897
# Promoter dREG bidirectionals	1072
# Intronic dREG bidirectionals	2125
# Intergenic dREG bidirectionals	543
Part of Tfit master merge?	True
Part of dREG master merge?	True