DBNascent | Samples

SRR1806549 Sample ID 88

**Data sources**
Paper identifier	Andrade2015dna
SRP accession	SRP055100
GEO accession	GSE65985
Samples	SRR1806549

**Technical details**
QC score	2
Replicate	2
Protocol	Bru-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	53 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	265 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HF1
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	bromouridine (2 mM)	-30 min	0 min	30 min
treatment	ultraviolet C (1 J/m^2/s)	-1820 s	-1800 s	20 s

**Extended notes**
Pipeline info and software versions
Sample notes	BrU-seq
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	52
Read depth after trimming	22,218,961
Duplication proportion	0.64554
Proportion reads mapped	0.9731
Exon/intron ratio	3.17329
Unique read proportion	0.40392

**Bidirectionals**
Total # Tfit bidirectionals	12053
# Promoter Tfit bidirectionals	3418
# Intronic Tfit bidirectionals	6089
# Intergenic Tfit bidirectionals	1670
Total # dREG bidirectionals	6110
# Promoter dREG bidirectionals	2197
# Intronic dREG bidirectionals	2726
# Intergenic dREG bidirectionals	910
Part of Tfit master merge?	True
Part of dREG master merge?	True